Computational methodology for protein interaction analysis

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Computational methodology for protein interaction analysis

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Problem

X-ray crystallography and nuclear magnetic resonance are two of the techniques recommended and used to characterize protein structures and complexes in studies. However, a significant portion of the proteins cannot be analyzed by such methods. Currently, there is a limitation of methodologies that allow the analysis of protein-protein interaction on a large scale. Mapping the epitope interaction region, i.e., the area of the antigen molecule that binds to cell receptors and antibodies, is a challenging task, and drug development depends on this mapping.

Solution

The technology proposes a method that, when applied to protein mixtures and subsequent analysis by the mass spectrometry technique, allows the characterization of protein complexes, epitope mapping, and protein-protein interaction on a large scale. Interaction identifications are performed using software developed by our team. In this way, the solution functions as an alternative to conventional methods of structural biology analysis.