Probe For Diagnosing Yellow Fever In Human and Animal Tissues.

The developed probe aims to improve the post-mortem diagnosis of yellow fever (YF) in human and animal tissues, through the colorimetric in situ hybridization (CISH) technique, replacing immunohistochemistry (IHC). 

While IHC is a commonly used test, its standardization across laboratories and the availability of commercial primary antibodies are limited. There are currently no commercially available antibodies or probes for IHC or CISH that detect YF virus in tissues. The Brazilian government depends on a remaining stock of a previously produced antibody, with no possibility of reproduction. 

Use of a nucleic acid probe for post-mortem diagnosis of yellow fever in human and animal tissues using the colorimetric in situ hybridization technique to replace the immunohistochemistry technique. The new technique is more sensitive and specific than the IHC technique currently used. The new probe will improve the specificity and sensitivity of AF post-mortem diagnosis compared to IHC.The probe may be sold separately or coupled to a CISH detection kit. 

Commercialization of the probe's know-how or industrial secret to a company in the pathological anatomy sector.